Cellular Pathology of Atherosclerosis

Q: What is the clinical evidence from this study?

Population: Atherosclerosis. Intervention: TNF-alpha and TGF-beta1 vs. Either agent in isolation. Primary outcome: Platelet adhesion.

Q: What does this research mean for the field?

Activation of endothelial cells by combined TNF-alpha and TGF-beta1 promotes high levels of stable platelet adhesion under low-shear conditions via a von Willebrand factor matrix requiring GPIb-IX-V and alpha(IIb)beta3 integrin receptors. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

Samantha Tull; Steve I. Anderson; Sascha C. Hughan; Steve P. Watson; Gerard B. Nash; G. Ed Rainger

doi:10.1161/01.res.0000198386.69355.87

December 2, 2005Circulation ResearchOpen Access

Cellular Pathology of Atherosclerosis

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Key Points

To investigate the cellular mechanisms driving pathological platelet adhesion to endothelial cells within an in vitro model of atherogenesis.
Cocultured endothelial cells with smooth muscle cells or treated endothelial cells alone with combinations of TNF-alpha and TGF-beta1.
Assayed platelet adhesion under flow conditions using receptor-blocking antibodies against GPIb-IX-V and alpha(IIb)beta3, alongside platelet activation inhibitors.
Visualized the endothelial surface matrix and von Willebrand factor assembly via confocal microscopy across wall shear rates below 400 s^-1.
Endothelial activation with both TNF-alpha and TGF-beta1 markedly increased platelet adhesion compared with treatment by either cytokine alone.
Stable platelet binding required intact platelet activation and was eliminated by blocking GPIb-IX-V, alpha(IIb)beta3 integrin, prostacyclin, or theophylline.
Activated endothelial cells assembled an extensive von Willebrand factor matrix capable of supporting sustained platelet adhesion under low shear rates (<400 s^-1).

PICO

P

Population

Atherosclerosis

I

Intervention / Comparator

TNF-alpha and TGF-beta1 vs Either agent in isolation

O

Primary Outcome

Platelet adhesion

Abstract

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Cite This Study

Tull et al. (2005) studied Atherosclerosis. TNF-alpha and TGF-beta1 vs. Either agent in isolation was evaluated on Platelet adhesion.

synapsesocial.com/papers/6a70181078a11c550e09e5ef https://doi.org/10.1161/01.res.0000198386.69355.87

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Cellular Pathology of Atherosclerosis

Key Points

PICO

Abstract

Discussion

Cite This Study

Cellular Pathology of Atherosclerosis

Key Points

PICO

Abstract

Discussion

Cite This Study

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