This in vitro study demonstrates that active streaming can be reconstituted using heavy meromyosin, actin, native tropomyosin, and Mg-ATP, providing insights into cytoplasmic streaming and muscle contraction mechanisms.
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In vitro reconstitution of active streaming is hypothesis-generating; leaves open relevance to cardiac sarcomere function in vivo.
Solutions containing heavy meromyosin, actin, native tropomyosin, and Mg-ATP exhibited streaming in horizontally placed glass microcapillaries. Up-hill streaming could also be observed when the capillaries were at an inclined position; this served for the clear distinction between active and passive streaming provided surface tension effects were eliminated. The presence of native tropomyosin and actin-activation of the ATPase activity of HMM were essential for the reconstitution of active streaming. The significance of the results for cytoplasmic streaming and muscle contraction is discussed.
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Tirosh et al. (1982) studied this question.
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