Inhibitor of DNA binding/differentiation 4 deficiency impairs hepatic fatty acid synthesis and is associated with epigenomic alterations in chromatin accessibility

mice compared to those of wild-type littermates. Furthermore, ID4 deficiency resulted in aberrant histone expression patterns. Integrative assay for transposase-accessible chromatin sequencing and RNA-sequencing analyses further revealed that ID4 deficiency induces chromatin closure at the promoter region of Srebf1 (encoding sterol regulatory element-binding protein), a master transcription factor upstream of Fasn (encoding FASN) and Acaca (encoding ACC1). Collectively, these findings indicate that ID4 functions as an important regulator of hepatic fatty acid metabolism by maintaining chromatin accessibility, rather than merely acting as a classical ID protein that regulates target gene transcription.