What question did this study set out to answer?

To isolate and characterize the variable region sequences and unique antigenic determinants of six murine IgA myeloma proteins specific for beta-(1 -> 6)-D-galactan.

November 1, 1973The Journal of Experimental MedicineOpen Access

SIX BALB/c IgA MYELOMA PROTEINS THAT BIND ß-(1 → 6)-D-GALACTAN

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To isolate and characterize the variable region sequences and unique antigenic determinants of six murine IgA myeloma proteins specific for beta-(1 -> 6)-D-galactan.
Purified six BALB/c mouse IgA myeloma proteins using galactoside-BSA-Sepharose affinity chromatography.
Determined partial N-terminal amino acid sequences of kappa light chains (through Cys23) and heavy chains (through residue 30) using automated sequencing.
Assessed unique individual antigenic determinants across all six proteins via hemagglutination inhibition assays.
All six kappa light chains displayed identical partial V(K) amino acid sequences, defining a unique murine V(K) subgroup distinct from 25 previously known subgroups.
Heavy chain partial sequences showed high conservation with only two variations: an isoleucine substitution for leucine at position 5 in one protein and an unidentified residue at position 19 in another.
Hemagglutination inhibition confirmed that despite near-identical partial sequences, each of the six proteins possessed a unique individual antigenic determinant.

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Implication

Laboratory study demonstrates shared variable domains and unique antigenic determinants in murine galactan-binding myeloma proteins, indicating subtle sequence variation governs specificity.

Cite This Study

Rudikoff et al. (1973) studied this question.

synapsesocial.com/papers/6a7021926c240de38cdbb08c https://doi.org/10.1084/jem.138.5.1095

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Implication

Laboratory study demonstrates shared variable domains and unique antigenic determinants in murine galactan-binding myeloma proteins, indicating subtle sequence variation governs specificity.

SIX BALB/c IgA MYELOMA PROTEINS THAT BIND ß-(1 → 6)-D-GALACTAN

Key Points

Discussion

Implication

Cite This Study

SIX BALB/c IgA MYELOMA PROTEINS THAT BIND ß-(1 → 6)-D-GALACTAN

Key Points

Discussion

Implication

Cite This Study

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