Highly Sensitive Multiplexed Heavy Metal Detection Using Quantum-Dot-Labeled DNAzymes

We developed highly sensitive and specific nanosensors based on quantum dots (QDs) and DNAzyme for multiplexed detection of heavy metal ions in liquid. The QDs were coated with a thin silica layer for increased stability and higher quantum yield while maintaining a relatively small size for highly efficient energy transfer. The QD-DNAzyme nanosensors were constructed by conjugating quencher-labeled DNAzymes onto the surface of carboxyl-silanized QDs. In the presence of metal ions, the emission is restored due to the cleavage of DNAzymes. The detection could be completed within 25 min with a single laser excitation source. The detection limit of 0.2 and 0.5 nM was experimentally achieved for Pb(2+) and Cu(2+), respectively, which is a 50- and 70-fold improvement over the recent results obtained with dye molecules. Multiplexed detection was also demonstrated using two different colors of QDs, showing negligible cross-talk between the Pb(2+) detection and Cu(2+) detection.