Combined strategy for suppressing breast carcinoma MCF-7 cell lines by loading simvastatin on alpha lipoic acid nanoparticles

BACKGROUND: Augmentation of simvastatin (SMV) cytotoxicity in breast carcinoma cell lines MCF-7, by: improvement of cellular uptake and loading on alpha lipoic acid (ALA). METHODS: In this study, SMV was loaded on ALA nanoparticles and characterized for surface morphology, SMV entrapment efficiency percent (%EE), zeta potential and release profile. Cellular viability, morphology and uptake and DNA fragmentations were analyzed as a hallmark of cellular apoptosis. RESULTS: was decreased to 22.2 ± 2.4 µM while raw SMV was 49.3 ± 6.6 µM. Cellular uptake of SMV-ALA nanoparticles was increased by about 3- and 2-folds after 2 and 4 h, respectively. DNA fragments confirmed the apoptosis property of ALA, which is associated with SMV cytotoxicity. CONCLUSION: This study suggests evidence that SMV loaded on ALA nanoparticles increases the MCF-7 cellular uptake and cytotoxic effects induced by SMV as revealed by significantly enhanced cell death rates in MCF-7 cells. These findings demonstrate that ALA induces cell death, which makes the combination a candidate for tumor therapy.