By means of the Edman phenylthiohydantoin method, the N-terminal sequences in the peptide chains of several human hemoglobins have been found to be as follows: α^A and α^F, val-leu-ser-pro-ala-aspNH_2-; β^A, val-his-leu-thr-pro-glu-; β^S, val-his-leu-thr-pro-val-; and γ^F, gly-his-phe.
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Shelton et al. (1960) studied this question.