Analytical and preparative high-speed countercurrent chromatography was successfully used for the separation of hydroxyanthraquinones from traditional Chinese medicine such as Rheum officinale Baill (Dahuang) and Polygonum cuspidatum Sieb. Et Zucc (Huzhang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion, and aloe-emodin were isolated each at over 98% purity.
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Yang et al. (2001) studied this question.
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