Quercetin Based Standardization Of Polyherbal Anti-Gout Remedy And İts Molecular Docking Study Against Anti-Gout And Anti-İnflammatory Protein Targets

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A five-herb containing traditional homemade medicine is extensively used to treat gout but has not been standardized for quercetin content. Therefore, the current study describes a reversed-phase liquid chromatographic method for quercetin determination in traditional herbal remedy. The elution was carried out using aqueous 2.0% acetic acid, acetonitrile and tetrahydrofuran (55:40:5, V/V/V) as mobile phase at flow rate of 0.8 mL/min and detection was performed using diode array detector operated at 370 nm. The response of the detector was linear in the range investigated (2.5-160.0 µg/mL) with R2 = 0.996. Results of recovery (98.26-103.22%, SD<5%), intraday accuracy and precision (94.68-104.08%, RSD<5%) and interday accuracy and precision (92.31-104.92%, RSD<5%) showed that the method was reliable, repeatable and reproducible, hence may be used for determination of quercetin in herbal remedy. The medicine contained 0.2425 mg/g quercetin. The molecular interactions of this marker compound were also studied against anti-gout and anti-inflammatory protein targets. Hence, the developed RP-HPLC method may be used to produce standardized anti-gout medicine for quercetin content. Moreover, the molecular interactions help in understanding underlying mechanism of action of this marker compound against gout.