Synthesis of Substrates Specific for Human Spleen Fibrinolytic Proteinase (Sfp)

Two peptide anilides, Suc‐Tyr‐Leu‐Val‐pNA (III) and Ac‐Ser‐His‐I.eu‐Val‐pNA (V) were synthesized by the conventional method with the object of obtaining specific substrates for human spleen fibrinolytic proteinase (SFP). The K cat /K m values for hydrolysis of HI and V by SFP were 22 64 7 and 2 700, respectively. On the contrary, the K cat /K m values for hydrolysis of III and V by porcine pancreatic elastase were 21 and 9, respectively.