Norepinephrine, unlike ATP, induces all‐or‐none increase in cytosolic calcium in thyroid cells

The mechanism of action of norepinephrine and ATP has been analyzed in single FRTL5 cells (a normal thyroid cell line), loaded with the fluorescent Ca2+ probe Fura2. ATP increased the cytosolic Ca2+ in an apparently concentration-dependent manner with a maximal effect at 10 microM (413 +/- 26% over basal levels of 135 +/- 7 nM). In contrast, the norepinephrine-induced increase (198 +/- 5% over basal) was concentration independent in individual cells, the minimal effective concentration being 1 nM. However, the number of cells responding to norepinephrine was concentration dependent. The ATP-induced Ca2+ rise was biphasic, consisting of a rapid rise (2-4 s, 252 +/- 15%), resembling the effect of norepinephrine, followed by a slower and longer component, which reached a plateau in 0.5-2 min. The second component appeared to be related to the opening of a channel, since it required extracellular Ca2+ and was abolished by SC38249, an inhibitor of the second-messenger-operated and voltage-operated channels. Moreover, it was inhibited by 4 beta-phorbol 12-myristate 13-acetate, suggesting that protein kinase C might be involved in the modulation of this Ca2+ channel.