Why the study?
Does SERCA2a gene transfer improve contractile function in isolated cardiomyocytes from failing human hearts?
Population
Isolated human ventricular myocytes from 10 patients with end-stage heart failure
Comparison
Gene transfer to overexpress SERCA2a vs Failing cardiomyocytes not overexpressing SERCA2a
Design
Preclinical
Authors
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Supports SERCA2a gene therapy development in heart failure; leaves open translation from animal cardiomyocyte models to patients.
Does SERCA2a gene transfer improve contractile function in isolated cardiomyocytes from failing human hearts?
SERCA2a gene transfer restores contractile function and calcium handling in failing human cardiomyocytes, providing preclinical support for gene-based therapies in heart failure.
Monte et al. (1999) studied this question.