Lack of cuprizone-induced demyelination in the murine spinal cord despite oligodendroglial alterations substantiates the concept of site-specific susceptibilities of the central nervous system

Figure S1. Photomicrographs of corpus callosum and spinal cord during cuprizone feeding. Comparison of myelination between corpus callosum and spinal cord white and grey matter using Luxol fast blue/cresyl violet staining (LFB; A, A′, B, B′) and different myelin protein-specific antibodies (C–E) in SJL mice. Myelination of the corpus callosum using LFB staining, control animal with normal myelination (A). Cuprizone-fed animal with severe loss of myelin after a 5-week cuprizone-feeding period (A′). Scale bars (A, A′), 100 μm. Myelination of ventral funiculi of cervical spinal cord using LFB staining, control animal with normal myelination (B). A cuprizone-fed animal after a 5-week cuprizone-feeding period with normal myelination (B′). CNPase (CNP), myelin basic protein (MBP), proteolipid protein (PLP) immunohistochemistry of control animals (C–E; control) and cuprizone-fed animals (C′–E′; CPZ) after 5-week cuprizone feeding. Control and cuprizone-fed animals displayed no differences regarding myelination in the spinal cord sections. Scale bars (B–D), 200 μm. Figure S2. Quantification of apoptosis in cuprizone-fed (CPZ) SJL mice using TUNEL assay. TUNEL-positive cells with apoptotic morphology (arrows) in the corpus callosum (A; 5-week cuprizone treatment; scale bar, 200 μm; insert: oil immersion, scale bar, 20 μm) and the thoracic spinal cord white matter (B; 1-week cuprizone treatment; oil immersion; scale bar, 20 μm). After 1- and 5-week cuprizone treatment, SJL mice showed significantly increased numbers of TUNEL-positive cells in the corpus callosum compared to controls (C). In the cervical spinal cord of cuprizone-fed SJL mice, a significant increase of TUNEL-positive cells were detected only at week 1 in cuprizone-fed mice compared to controls. Note low numbers of TUNEL-positive cells at later time points (D). Graphs display data as median with range (*P ≤ 0.05). Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.