Greener Synthetic and Nanotechnological Bridge: Theoretical and Practical Biological Approach Toward the Bactericidal Dual Action Mechanism of Azomethine–Nanosilver Hybrid

An efficient, clean, cheap, and quick procedure for the condensation of aromatic primary amine, namely, 4-aminophenazone, with different aryl carbonyls to produce azomethines under solvent-free and catalyst-free conditions at room temperature has been described. Their structures were confirmed by 1H-nuclear magnetic resonance (NMR), infrared (IR), mass spectra, and elemental analysis. The corresponding azomethines has been screened virtually using bioinformatical parameters on the basis of hypothetical antibacterial pharmacophore structure designed to interact with both of gram-positive bacteria and gram-negative bacteria. The virtually screened molecules were subjected to screening against certain strains of gram-positive and gram-negative bacteria. Based on the results of initial screening, the bactericidal action of some selected compounds has been increased by hybrid coupling with silver nanoparticles. The results thus obtained are discussed.