A Study on the Process of Polysaccharide Extraction From Myxopyrum Smilacifolium (Wall.) Blume Roots

Myxopyrum smilacifolium (Wall.) has been demonstrated to contain many bioactive compounds, including polysaccharides, saponin, and flavonoids. This study aimed to determine the effects of the single-factor model on the attraction process as a basis for optimal performance. The obtained extract will be evaluated for its antioxidant capacity. The results of the single-factor when extracting the total polysaccharide from Myxopyrum smilacifolium (Wall.) showed the effects of the solvent concentration, solvent/material ratio, extraction time, and temperature on the extraction process with the corresponding results: ethanol 60% (v/v), 20/1 (ml/g), 75 minutes and 70°C respectively. The optimal conditions have been found for the total polysaccharide extraction from Myxopyrum smilacifolium (Wall.): ethanol concentration 66.58%, solvent/material ratio 20.46/1 (ml/g), extraction time 76.77 min, extraction temperature 70°C, respectively. Under the conditions, the total polysaccharide content of the extract reached 3.34937 mg/g of the raw material of Myxopyrum smilacifolium (Wall.) leaves. The extract from Myxopyrum smilacifolium (Wall.) gave an IC50 value of 522.56 ± 13.67 (µg/ml) when evaluating its antioxidant activity.