Production of a cholinesterase-solubilizing factor from a Cytophaga sp. by continuous cultivation

Cytophaga sp. (NCMB 1314) produces a factor capable of solubilizing cholinesterase from body muscle of plaice. A simple dialyzable medium supporting bacterial growth and production of the cholinesterase-solubilizing factor has been developed. The optimal conditions for extracellular production of the factor have been investigated. Either the whole culture or a cell-free preparation can be used to liberate structurally bound cholinesterase from body muscle of plaice.