Several strain of bakers' yeast that accumulate only C27 sterols were analyzed for sterol methyltransferase activity, with no activity being found. Cholesta-5,7,22,24-tetraene-3beta-ol, one of the mutants' sterol products, was found to be an unacceptable substrate for in vitro transmethylation.
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Bailey et al. (1976) studied this question.
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