Use of Cibracon Blue-Sepharose 6B to Detect Modifications of the Non-allosteric 6-Phosphofructokinase from Escherichia Coli K-12

In crude cell-free extracts of aerobically grown E. coli K-12, the non-allosteric form of 6-phosphofructokinase has a tetrameric molecular weight of 140000 with a low affinity « 5%) for the blue dextran chromophore-Cibracon Blue. The allosteric form has the same tetrameric molecular weight, but possesses a strong affinity for the blue dextran chromophore.