We report a simple and low-cost strategy that allows the sequential and site-specific assembly of a dehydrogenase-based multi-enzyme cascade for methanol oxidation on the yeast surface using the high-affinity interactions between three orthogonal cohesin-dockerin pairs. The multi-enzyme cascade showed 5 times higher NADH production rate than the non-complexed enzyme mixture, a result of efficient substrate channeling.
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Liu et al. (2013) studied this question.