A method was developed for the quantitative determination of rapeseed glucosinolates as the desulfo derivatives, using liquid chromatography. Glucosinolates were desulfated at 37°C with the enzyme aryl sulfatase in Tris buffer, pH 8.0. All glucosinolates present in rapeseed were separated in 30 min on a Waters C18 Z-module using an acetonitrile gradient at 4 mL/min. Recoveries of benzyl, 4-hydroxybenzyl, and allylglucosinolates added to plant extracts were quantitative.
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Sang et al. (1984) studied this question.