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Summary. 1. The total content of contractive substance in human sperm was determined from 155 specimens of sperm by titration on rabbit intestine in vitro, with prostaglandin as the standard. The content varied greatly in different specimens, but contractive substance was met with in all of them. 2. In certain specimens the factor which is active on rabbit intestine consists – apart from prostaglandin and choline – of at least one other substance, which leads to a very rapid increase in tonus and is indifferent to atropine. 3. There is no correlation between the total content of contractive substance and the motility and life of the spermatozoa.
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Jan Asplund (1947) studied this question.
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