What question did this study set out to answer?

To determine whether brief exposure to low levels of ethanol stimulates the synthesis of plasminogen activators and enhances fibrinolytic activity in cultured human endothelial cells.

April 1, 1998Alcoholism Clinical and Experimental Research

Alcohol‐Induced Upregulation of Plasminogen Activators and Fibrinolytic Activity in Cultured Human Endothelial Cells

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To determine whether brief exposure to low levels of ethanol stimulates the synthesis of plasminogen activators and enhances fibrinolytic activity in cultured human endothelial cells.
Preincubated cultured human saphenous vein endothelial cells (HSVECs) with 0.01% to 0.1% (v/v) ethanol for 5 to 120 minutes at 37°C.
Washed and incubated cells alcohol-free for 8 and 24 hours, evaluating t-PA and u-PA mRNA via RT-PCR and secreted antigen levels via ELISA.
Quantified net endogenous surface-localized fibrinolytic activity (plasmin generation) using 125I-Glu-plasminogen activation after 24 hours.
A 5 to 30 minute exposure to alcohol increased t-PA antigen from 14.2 ± 1.7 to 25.4 ± 5 ng/ml and u-PA antigen from 15 ± 0.8 to 46.4 ± 1.3 ng/ml (approximately 3-fold increases).
Ethanol exposure led to an approximately 2-fold increase in both t-PA and u-PA mRNA levels relative to controls.
Surface-localized fibrinolytic activity rose significantly by more than 2.5-fold, from 96 ± 2.8 fmol/well in controls to 255 ± 42 fmol/well with alcohol.

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Implication

In vitro study demonstrates brief alcohol exposure upregulates plasminogen activators in human endothelial cells, suggesting a biological mechanism for moderate drinking's cardioprotective effects.

Cite This Study

Aikens et al. (1998) studied this question.

synapsesocial.com/papers/6a70670be36a167817e22d4a https://doi.org/10.1111/j.1530-0277.1998.tb03663.x

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Implication

In vitro study demonstrates brief alcohol exposure upregulates plasminogen activators in human endothelial cells, suggesting a biological mechanism for moderate drinking's cardioprotective effects.

Alcohol‐Induced Upregulation of Plasminogen Activators and Fibrinolytic Activity in Cultured Human Endothelial Cells

Key Points

Discussion

Implication

Cite This Study

Alcohol‐Induced Upregulation of Plasminogen Activators and Fibrinolytic Activity in Cultured Human Endothelial Cells

Key Points

Discussion

Implication

Cite This Study

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