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Four strains of Neisseria meningitidis were studied during serial passage. Upon subcultivation, two of them lost the ability to liberate endotoxin. Ultrastructurally, the two parent endotoxin liberating strains exhibited quantitatively more free cell wall membranes and blebs in the medium than their non-liberating variants. Similarly, the endotoxin-releasing original strains exhibited higher sulfonamide resistance than their variants, and had markedly more sticky cells, which showed pronounced adherence to the surfaces of plastic and heated blood agar.
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Andersen et al. (1981) studied this question.
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