A procedure is described to separate mercury from, mammalian tissues so that a previously devised photometric method of analysis for submicrogram amounts of mercury can be used with precision for such samples. Mercury is first separated from tissue by a cold digestion in hydrochloric acid-sodium nitrate; it is then collected on an anion exchange column and eluted with thiourea solution. Finally it is collected in cadmium sulfide, volatilized at 550° C., and determined photometrically by using a General Electric germicidal ultraviolet intensity meter. Results of analyses of rat and mouse tissues are presented, and the usefulness of the procedure for industrial hygiene is discussed.
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Toribara et al. (1968) studied this question.
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