Type I Pili Mediate Gram-negative Bacterial Adherence to Intact Tracheal Epithelium

We have investigated the role of pili in mediating gram-negative bacterial adherence to an intact tracheal epithelium. Type 1 pili, but not P or Pseudomonas pili, markedly increased bacterial adherence. The adherence-promoting effect of Type 1 pili was due to the mannose-binding Type 1 pili adhesin, as both alpha-methyl mannoside and concanavalin A blocked adherence of Type 1 piliated bacteria. The Type 1 pili-binding site on tracheal epithelium appears to be a mannose-containing glycoprotein. Clearance of Type 1 piliated bacteria from the lung parenchyma was assessed by depositing the bacteria into a lobe; no difference in clearance rates between Type 1 and nonpiliated bacteria was present. Type 1 pili may enhance the ability of gram-negative bacteria to adhere to and colonize the lower respiratory tract.