6+ . The leaf powder was found to be stable for at least one year when kept in dry condition at room temperature. However, compositions of plant parts are known to vary due to various factors like variety, climate, soil, competition, diseases, etc. Therefore, the same batch of reagents should be used for preparing the calibration curve and analysis of samples. Table 3 lists other plant extracts that gave similar colour reaction as S. jambolanum. Therefore, it may be concluded that compounds responsible for the colour reaction are widely distributed. The S. jambolanum extract gave a deep purple colour with ferric iron, lemon yellow colour with titanium and a bluish colour with vanadate ion. A compound like quercetin or tannic acid may be involved in colour development.
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Hosseinnezhad et al. (2015) studied this question.
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