The Nucleotide Sequence of the GGG-specific Glycine Transfer Ribonucleic Acid of Escherichia coli and of Salmonella typhimurium

The primary nucleotide sequence of tRNAggggly (glycine tRNA specific for the codon GGG) from Escherichia coli and Salmonella typhimurium has been determined. The E. coli tRNA sequence is [see PDF for sequence] where U at position 8 is most likely 4-thiouracil. The sequence of the S. typhimurium tRNAggggly is identical with that of E. coli except that no evidence for the modification of U at position 8 has been found. In addition, a portion of the S. typhimurium tRNAggggly has a 2'-O-methyl modification of the G at position 17. Some unusual features of the tRNA structure are discussed.