Coping with and Recovering from Hydroxyurea-induced Replication Fork Arrest in Budding Yeast

During S phase, the activity of large numbers of replicons must be regulated and coordinated to ensure that thegenome is replicated accurately and quickly (Pasero andSchwob 2000). Replication origins are not all activatedtogether at the beginning of S phase, but they are insteadactivated at different times throughout S phase accordingto a predetermined cell-type-specific program (Fangmanand Brewer 1992; Brewer et al. 1993). Thus, at any giventime, there may be initiation, elongation, and terminationreactions occurring simultaneously in the same nucleus...