Effect of ammonium nutrition on uptake and metabolism of nitrate in wheat.

Wheat plants 1 month old grown in nitrate culture were exposed for 2 days to media containing 4 meq NO3 and 0-4 meq NH4/l. Where solutions contained both forms of N the uptake of NH4 exceeded that of NO3 even at the lowest NH4 level. NH4 metabolism predominated over NO3 assimilation and resulted in a higher free amino acid content, a lower carboxylate content and lower nitrate reductase activity in the tops than with NO3 alone. NH4 inhibited nitrate reductase activity whereas amino acids did not. The fall in activity of this enzyme in wheat tops caused by NH4 nutrition could not be related to the low level of tissue NH4 and it was suggested that changes in the redox situation in the cells might be involved. (Abstract retrieved from CAB Abstracts by CABI’s permission)