Urinary Bladder Blood Flow Changes During the Micturition Cycle in a Conscious Pig Model

No AccessJournal of UrologyInvestigative Urology1 Nov 1996Urinary Bladder Blood Flow Changes During the Micturition Cycle in a Conscious Pig Model J.E. Greenland and A.F. Brading J.E. GreenlandJ.E. Greenland and A.F. BradingA.F. Brading View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65553-8AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To develop a method using laser Doppler flowmetry in conscious pigs, which allows the accurate simultaneous measurement of cystometric and cardiovascular parameters together with changes in vesical blood flow. The animal model was then used to investigate the changes in blood flow in the urinary bladder which occur during the micturition cycle. Materials and Methods: Seven large white female pigs were subjected to chronically implanted vascular access and urodynamic catheters as well as an intramural vesical laser Doppler probe. The animals underwent repeated conscious urodynamics with simultaneous measurement of cardiovascular, urodynamic and vesical blood flow parameters. Results: The model shows both compliant and low-compliance behavior and allows greater investigation of the effects of intravesical pressure on blood flow. Blood flow is not altered, during compliant filling and voiding transiently decreases flow to 38 percent of resting levels, with a rapid return to normal. Low-compliance filling results in a progressive fall in blood flow to a minimum of 45 percent of normal. At all times an inverse relationship between intravesical pressure and blood flow is maintained. Conclusions: The pig model proved to be well suited to the experimental conditions and provided reproducible results. The principal determinant of blood flow within the wall of the bladder is the pressure within its lumen. During normal filling the blood supply of the bladder is able to adapt to the large increase in surface area which occurs, maintaining blood flow until the pressure increases. References 1 : An experimental study of the vesical circulation during distension and in cystitis. J. Pathol. Bacteriol.1953; 66: 79. Google Scholar 2 : A study of the bladder blood flow during distension in rabbits. Br. J. Urol.1975; 47: 67. Google Scholar 3 : Effect of distension on blood flow in dog's urinary bladder. Invest. Urol.1974; 12: 210. Google Scholar 4 : Changes in canine bladder perfusion with distension. Invest. Urol.1977; 15: 149. Google Scholar 5 : Rate of urinary bladder blood flow evaluated by 133Xe washout and radioactive microspheres in pigs. Urol. Res.1991; 19: 387. Google Scholar 6 Bellringer, J. F., Ward, J. and Fry, C. H.: Evaluation of a laser Doppler technique for the measurement of bladder blood flow in the anaesthetised rabbit. Proceedings International Continence Society, p. 89, 1993. Google Scholar 7 : Effect of bladder filling and contraction on bladder microcirculation. Neurourol. Urodyn.1993; 12: 400. Google Scholar 8 : Bladder vasodilatation and release of vasoactive intestinal polypeptide from the urinary bladder of the cat in response to pelvic nerve stimulation. J. Urol.1987; 138: 671. Link, Google Scholar 9 : Impaired bladder perfusion in interstitial cystitis: a study of blood supply using laser Doppler flowmetry. J. Urol.1993; 149: 890. Link, Google Scholar 10 : Urinary bladder blood flow. I. Comparison of clearance of locally injected 99m-technetium pertechnate and radioactive microsphere technique in dogs. Urol. Res.1990; 18: 223. Google Scholar 11 : Effects of nerve stimulation on blood flow in the urinary bladder, urethra and pelvic floor in the dog. J. Urol.1993; 150: 1945. Abstract, Google Scholar 12 : Simultaneous assessment of red cell perfusion in skeletal muscle by laser Doppler flowmetry and video microscopy. Int. J. Microcirc. Clin. Exp.1985; 4: 397. Google Scholar 13 : Evaluation of laser-Doppler flowmetry as a measure of tissue blood flow. J. Appl. Physiol.1986; 61: 666. Google Scholar 14 : An experimental model of detrusor instability in the obstructed pig. Br. J. Urol.1985; 57: 292. Google Scholar 15 : A histochemical and immunohistochemical study of the autonomic innervation of the lower urinary tract of the female pig. Is the pig a good model for the human bladder and urethra? J. Urol.1989; 141: 414. Google Scholar 16 : Conscious minipig model for evaluation the lower urinary tract. Neurourol. Urodyn.1994; 13: 147. Google Scholar 17 : The structure and innervation of smooth muscle in the wall of the bladder neck and proximal urethra. Br. J. Urol.1975; 47: 549. Google Scholar 18 : The unstable bladder: towards a common mechanism. Br. J. Urol.1994; 73: 3. Google Scholar 19 : The arterial supply of the male urinary bladder. Br. J. Urol.1952; 24: 64. Google Scholar 20 : Microangiography of the bladder in health. Br. J. Urol.1981; 53: 237. Google Scholar University Department of Pharmacology, Oxford University and the Department of Urology, Churchill Hospital, Headington, Oxford, United Kingdom.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byBRATSLAVSKY G, KOGAN B, MATSUMOTO S, ASLAN A and LEVIN R (2018) Reperfusion Injury of the Rat Bladder is Worse Than IschemiaJournal of Urology, VOL. 170, NO. 5, (2086-2090), Online publication date: 1-Nov-2003.Kozlowski R, Siroky M, Krane R and Azadzoi K (2018) Regulation Of Blood Flow And Microcirculation Resistance In Rabbit BladderJournal of Urology, VOL. 168, NO. 4 Part 1, (1608-1614), Online publication date: 1-Oct-2002.Kershen R, Azadzoi K and Siroky M (2018) Blood Flow, Pressure and Compliance in the Male Human BladderJournal of Urology, VOL. 168, NO. 1, (121-125), Online publication date: 1-Jul-2002.PAREKH M, LOBEL R, O'CONNOR L, LEGGETT R and LEVIN R (2018) PROTECTIVE EFFECT OF VITAMIN E ON THE RESPONSE OF THE RABBIT BLADDER TO PARTIAL OUTLET OBSTRUCTIONJournal of Urology, VOL. 166, NO. 1, (341-346), Online publication date: 1-Jul-2001.BRATSLAVSKY G, KOGAN B and LEVIN R (2018) URETHRA IS MORE SENSITIVE TO ISCHEMIA THAN BLADDER: EVIDENCE FROM AN IN VITRO RAT STUDYJournal of Urology, VOL. 165, NO. 6 Part 1, (2086-2090), Online publication date: 1-Jun-2001.Greenland J and Brading A (2018) THE EFFECT OF BLADDER OUTFLOW OBSTRUCTION ON DETRUSOR BLOOD FLOW CHANGES DURING THE VOIDING CYCLE IN CONSCIOUS PIGSJournal of Urology, VOL. 165, NO. 1, (245-248), Online publication date: 1-Jan-2001.SCHRÖDER A, CHICHESTER P, KOGAN B, LONGHURST P, LIEB J, DAS A and LEVIN R (2018) EFFECT OF CHRONIC BLADDER OUTLET OBSTRUCTION ON BLOOD FLOW OF THE RABBIT BLADDERJournal of Urology, VOL. 165, NO. 2, (640-646), Online publication date: 1-Feb-2001.LIEB J, CHICHESTER P, KOGAN B, DAS A, LEGGETT R, SCHRÖDER A and LEVIN R (2018) RABBIT URINARY BLADDER BLOOD FLOW CHANGES DURING THE INITIAL STAGE OF PARTIAL OUTLET OBSTRUCTIONJournal of Urology, VOL. 164, NO. 4, (1390-1397), Online publication date: 1-Oct-2000.PONTARI M, HANNO P and RUGGIERI M (2018) COMPARISON OF BLADDER BLOOD FLOW IN PATIENTS WITH AND WITHOUT INTERSTITIAL CYSTITISJournal of Urology, VOL. 162, NO. 2, (330-334), Online publication date: 1-Aug-1999. Volume 156Issue 5November 1996Page: 1858-1861 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information J.E. Greenland More articles by this author A.F. Brading More articles by this author Expand All Advertisement PDF downloadLoading ...