The IVIP protocol enables fast, simple, and reproducible preparations of intact and highly purified retroviral particles devoid of detectable extracellular vesicle contaminants.
IVIP enables rapid purification of intact retroviruses; leaves open its adoption in virology research pending validation.
, 2018 ). This technique, that we call intact virion immunoprecipitation (IVIP), enabled us to characterize the accessibility of epitopes on the surface of these retroviruses and assess the orientation of the virus-encoded integral membrane protein Glycogag (gPr80) in the viral envelope. Proper implementation of this protocol enables fast, simple and reproducible preparations of intact and highly purified retroviral particles devoid of detectable EV contaminants.
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Renner et al. (2018) studied this question.
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