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A fluorometric method for the determination of aliphatic aldehydes using 1, 3-cyclohexanedione reagent and high-performance liquid chromatography (HPLC) was developed.Straight-chain aldehydes of C3-C10 were reacted with 1, 3-cyclohexanedione at 60°C for 1 h and the fluorescent decahydroacridine derivatives2, 3) formed were determined by HPLC on an ODS column. The calibration curves for the aldehydes were linear in the range of 0-10 ng and the relative standard deviation at the 5 ng level was about 2.3%.This method was sensitive enough to measure the amount of aliphatic aldehydes, including 4-hydroxynonenal, generated by microsomal peroxidation in rat liver.
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Yoshino et al. (1986) studied this question.
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