The study demonstrates the successful construction of high-titer retroviral vectors capable of simultaneously expressing three or four genes, advancing gene transfer technology.
Facilitates multi-gene retroviral delivery; leaves open in vivo validation before any clinical translation.
We have combined the picornavirus foot-and-mouth disease virus (FMDV) 2A sequence and the internal ribosome entry sites (IRESes) from encephalomyocarditis virus (ECMV) and avian reticuloendotheliosis virus type A (REV-A) to construct tricistronic and tetracistronic vectors. All the polycistronic constructs show high titers and expression of the genes inserted. Clones have been obtained in which cells simultaneously express the three or four genes carried by the polycistronic vectors.
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Felipe et al. (2000) studied this question.
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