Excitation wavelength effects on hematoporphyrin (HP) and hematoporphyrin derivative (HPD, Photofrin II) fluorescence spectra, quantum yields and decays were comparatively studied in homo geneous (at different pH and substrate concentrations) and microheterogeneous (Triton X 100 micelles and Bovine serum albumin) aqueous environments, in which the porphyrins are expected to be present in different monomer/aggregate equilibria. The observed wavelength dependences of the fluorescence yield and of short lived contributions to the total fluorescence do not correlate to the porphyrin aggregation state obtained from absorption spectra. In micro-heterogeneous environ ment the influence of local interactions between the porphyrin and hydrophobic sites of the cosolutes on HP and HPD photophysics is demonstrated.
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Köhler et al. (1989) studied this question.
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