Genetic and biotechnological strategies to exploit plant cell walls for bioenergy production

• Comprehensive overview and functional interpretation of key genes regulating cellulose, hemicellulose, pectin, and lignin pathways • Multi-omics strategies to decode cell wall regulatory networks controlling plant cell wall biosynthesis and remodelling for bioenergy. • Advances in genetic engineering, genome editing, and epigenetic approaches to enhance biomass digestibility and saccharification efficiency. • Framework for designing the archetypal bioenergy crop that balances conversion efficiency, structural integrity, and environmental resilience. The plant cell wall is a complex and dynamic structure that gives plants strength and flexibility, supporting growth, development, and mediating responses to environmental stimuli. It also represents an important source of renewable lignocellulosic biomass for bioenergy production. The plant cell wall has been studied for years, but the processes controlling its construction, deconstruction, and remodeling remain largely unclear. Genetic technologies have revealed new details about the network of genes regulating the production and modification of cellulose, hemicellulose, pectin, and lignin. These methods are providing us with a more complete view of the complexity of cell wall metabolism. Furthermore, innovative biotechnological tools, such as CRISPR/Cas9 and other genetic engineering techniques, have been shown to facilitate cell wall degradation, promoting the release of fermentable sugars from the biomass. In this review, we summarize recent advances in understanding the molecular composition, biosynthesis, and remodeling of cell wall polymers, highlighting how combining multi-omics data with biotechnology can improve biomass yield, composition, and digestibility. We also discuss key challenges, such as the trade-off between increasing biomass saccharification value and plant performance. Knowledge gaps regarding the link between molecular regulation and field performance under changing environmental conditions remain to be filled. Combining genetic, biochemical, and systems biology perspectives, this review outlines a framework for designing “archetypal” bioenergy crops to balance process efficiency and environmental resilience, thus promoting the sustainable conversion of lignocellulosic biomass into bioenergy and bioproducts.