What question did this study set out to answer?

To determine whether the inhibitory effects of clinically used estrogens on human aortic smooth muscle cell biology and mitogen-activated protein kinase activity are mediated by estrogen receptors.

April 1, 2000Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

Clinically Used Estrogens Differentially Inhibit Human Aortic Smooth Muscle Cell Growth and Mitogen-Activated Protein Kinase Activity

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To determine whether the inhibitory effects of clinically used estrogens on human aortic smooth muscle cell biology and mitogen-activated protein kinase activity are mediated by estrogen receptors.
Cultured human aortic smooth muscle cells were treated with clinically used estrogens in the presence or absence of the estrogen receptor antagonist ICI 182,780.
Assessed DNA synthesis via [3H]thymidine incorporation, cell proliferation by cell counting, migration using a modified Boyden chamber, collagen synthesis via [3H]proline incorporation, and mitogen-activated protein kinase activity.
17beta-estradiol at physiological (10^-9 mol/L) and low concentrations (10^-8 to 10^-7 mol/L), along with estradiol valerate, estradiol cypionate, and estradiol benzoate, significantly inhibited DNA synthesis, proliferation, collagen synthesis, migration, and mitogen-activated protein kinase activity, whereas estrone, estriol, 17alpha-estradiol, and estrone sulfate did not.
The inhibitory actions were completely reversed by 100 micromol/L ICI 182,780, and the rank-order potency of the estrogens directly matched their binding affinity for estrogen receptors.

Authors

RDRaghvendra K. DubeyUniversity of Pittsburgh EJEdwin K. JacksonCross-Cutting Cardiology DGDelbert G. GillespieUniversity of Pittsburgh

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Implication

In vitro study reveals that select estrogens inhibit human aortic smooth muscle cell growth via estrogen receptors, suggesting hormone replacement benefits depend on receptor binding affinity.

Cite This Study

Dubey et al. (2000) studied this question.

synapsesocial.com/papers/6a70a0cc35aa2c282ce1f3bc https://doi.org/10.1161/01.atv.20.4.964

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Implication

In vitro study reveals that select estrogens inhibit human aortic smooth muscle cell growth via estrogen receptors, suggesting hormone replacement benefits depend on receptor binding affinity.

Clinically Used Estrogens Differentially Inhibit Human Aortic Smooth Muscle Cell Growth and Mitogen-Activated Protein Kinase Activity

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Discussion

Implication

Cite This Study

Clinically Used Estrogens Differentially Inhibit Human Aortic Smooth Muscle Cell Growth and Mitogen-Activated Protein Kinase Activity

Key Points

Discussion

Implication

Cite This Study

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