What question did this study set out to answer?

To assess the ability of the type II methanotroph Methylosinus trichosporium OB3b to degrade trichloroethylene and related chlorinated hydrocarbons.

December 1, 1989Applied and Environmental MicrobiologyOpen Access

Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b

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Authors

HTH C TsienUniversity of Minnesota GBGregory A. BrusseauNorthwestern University RHR S HansonResearch Triangle Park Foundation

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Implication

Laboratory study demonstrates rapid trichloroethylene breakdown by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase, suggesting effective bioremediation potential.

Key Points

To assess the ability of the type II methanotroph Methylosinus trichosporium OB3b to degrade trichloroethylene and related chlorinated hydrocarbons.
Cultured Methylosinus trichosporium OB3b in continuous and batch setups under low-copper conditions (≤0.25 µM copper sulfate) to induce soluble methane monooxygenase synthesis.
Verified soluble methane monooxygenase expression using Western blot analysis with specific purified enzyme antibodies.
Measured oxidation rates of trichloroethylene, methane, methanol, and chlorinated ethylenes.
Trichloroethylene was degraded at rates exceeding 1.2 mmol/h per g dry weight upon expression of soluble methane monooxygenase.
Growth in media containing 0.25 µM or less copper sulfate derepressed soluble methane monooxygenase synthesis, increasing trichloroethylene oxidation without altering specific rates of methane or methanol oxidation.
Cells expressing soluble methane monooxygenase also successfully degraded vinyl chloride, 1,1-dichloroethylene, cis-1,2-dichloroethylene, and trans-1,2-dichloroethylene.

Cite This Study

Tsien et al. (1989) studied this question.

synapsesocial.com/papers/6a70a822e5469ee92be15cf3 https://doi.org/10.1128/aem.55.12.3155-3161.1989

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Implication

Laboratory study demonstrates rapid trichloroethylene breakdown by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase, suggesting effective bioremediation potential.

Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b

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Implication

Key Points

Cite This Study

Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b

Discussion

Member takes

Implication

Key Points

Cite This Study

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