A new method for the determination of paeoniflorin and albiflorin in crude drug Shaoyao ( ?? ??, peony root) was established by the use of a rod-thin-layer chromatograph equipped with a flame ionization detector (rod-TLC-FID). The test solution was submitted to rod-TLC-FID method using a mixture of chloroform-methanol (5 : 1) as a developing solvent. The content of paeoniflorin and albiflorin was calculated from their calibration curves previously prepared using guaiacol glyceryl ether as an internal standard. The recovery rate of paeoniflorin and albiflorin was more than 95%. This method is considered to be useful for the chemical evaluation of Shaoyao.
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Yoshizaki et al. (1977) studied this question.
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