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June 1, 1989Journal of VirologyOpen Access

The palindromic LTR-LTR junction of Moloney murine leukemia virus is not an efficient substrate for proviral integration

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Population

Cells infected with recombinant viral constructs of Moloney murine leukemia virus

Comparison

Viral constructs carrying a selectable marker… vs Natural sequences at the ends of the genome

Design

Preclinical

Authors

LLL I LobelJMJohn E. MurphySGStephen P. Goff

Discussion

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Overview

Questions LTR-LTR junction efficiency for integration; leaves open linear DNA as the authentic retroviral precursor in animal models.

Structured PICO

P
Population
Cells infected with recombinant viral constructs of Moloney murine leukemia virus
I
Intervention
Viral constructs carrying a selectable marker and an extra copy of the LTR-LTR junction from a cloned circular provirus
C
Comparator
Natural sequences at the ends of the genome
O
Outcome
Efficiency of use of the introduced junction sequence during integration

The palindromic LTR-LTR junction is not an efficient substrate for proviral integration, suggesting a linear or more exotic DNA intermediate is the true precursor.

Cite This Study

Lobel et al. (1989) studied this question.

synapsesocial.com/papers/6a70b108f44fa9f079de620dhttps://doi.org/10.1128/jvi.63.6.2629-2637.1989
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Also Consider

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  1. 1Construction and Characterization of a Retroviral Vector Demonstrating Efficient Expression of Cloned cDNA Sequences1988 · 156 citations
  2. 2Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.1979 · 3,351 citations
  3. 3Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.1980 · 323 citations