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Fatty acid methyl esters (FAMES) were prepared from phospholipid extracts of beef, pork, lamb, chicken and turkey using four methylating procedures: BF 3 /methanol, KOH/BF 3 , direct transesterification with KOH, and a commercial preparation, Meth Prep II. Gas chromatographic analysis of the FAMES revealed the presence of dimethyl acetals (DMAs) in samples methylated with BF 3 /methanol or KOH/ BF 3 . These DMAs were evident when samples were analyzed on both polar and non‐polar columns. DMAs were not found in samples derivatized by direct alkali transesterification or by Meth Prep II.
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CRACKEL et al. (1988) studied this question.
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