Protocol for inducing varying TBI severity in a mouse model using a closed-head, weight-drop, impact-induced acceleration mechanism

Animal models of traumatic brain injury (TBI) are critical for understanding its complex neuropathology. Here, we present a protocol to induce varying TBI severities in mice using a closed-head, weight-drop model that includes an impact-induced acceleration mechanism. We describe steps for habituation with neurological severity score (NSS) equipment, assessing NSS baseline, performing anesthesia and TBI, assessing NSS post-injury, and analyzing data. This protocol requires no prior surgical intervention and is adaptable for rat studies. For complete details on the use and execution of this protocol, please refer to PhD Dissertation of Javier Allende Labastida 1 and Tang et al. 2 • Steps for implementing closed-head impact-acceleration TBI model • Instructions for assessing TBI severity using the neurological severity score • Guidance on setting up TBI and NSS apparatuses Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Animal models of traumatic brain injury (TBI) are critical for understanding its complex neuropathology. Here, we present a protocol to induce varying TBI severities in mice using a closed-head, weight-drop model that includes an impact-induced acceleration mechanism. We describe steps for habituation with neurological severity score (NSS) equipment, assessing NSS baseline, performing anesthesia and TBI, assessing NSS post-injury, and analyzing data. This protocol requires no prior surgical intervention and is adaptable for rat studies.