The study identifies a specific site of action for S1 nuclease on double-stranded circular DNA intermediates of an avian RNA tumor virus.
Reveals a nuclease-sensitive site in retroviral DNA circles; leaves open its role in replication or integration.
Circular viral DNA intermediates obtained from the quail tumor line, QT6, at 1 day after infection, were opened at one specific location by the single-strand specific nuclease, S1, of Aspergillus oryzae. This site was no longer accessible to the S1 nuclease when circles were first opened at another location with a restriction endonuclease.
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Hsu et al. (1978) studied this question.
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