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A method was developed for sex determination in two parrot species (Amazona a. aestiva and Amazona amazonica). A protocol is described for direct PCR amplification from parrot whole blood using primers designed for Xho I repetitive regions of chicken W-chromosome. A W-specific fragment (approximate size of 124 bp) was amplified only in six DNA-female samples. No amplification was observed in 19 male samples. Complete correspondence was observed between our sex identification results and those obtained with endoscopy, karyotype analyses, and behavioral observations. J. Exp. Zool. 280:217–219, 1998.
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Mattos et al. (1998) studied this question.
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