Acyl Dihydroxyacetone Phosphate

An unknown mitochondrial lipid which is labeled in vitro by incubation with γ-32P-adenosine triphosphate has been isolated. A series of degradations including hydrolysis, oxidation, and reduction of the 32P-lipid resulted in various labeled products which were then characterized by co-chromatography or by co-electrophoresis. Alkaline methanolysis resulted in the release of all radioactivity from the lipid as inorganic phosphate. On the basis of its chemical properties in comparison with synthetic palmitoyldihydroxyacetone phosphate, the lipid has been identified as an acyl dihydroxyacetone phosphate.