Fab'‐fragments derived from antibodies against β 2 ‐microglobulin will completely abolish the cytotoxic effect of antibodies against HL‐A serological determinants. This is the result of the Fab'‐fragments competing with the antibodies against the HL‐A antigens for binding to the lymphocyte surface. Reciprocally, it is shown that Fab'‐fragments against the HL‐A alloantigenic structures impeded the binding of Fab'‐fragments to cell surface bound β 2 ‐microglobulin. Fab'‐fragments against β 2 ‐microglobulin inhibit considerably the mixed leucocyte culture reaction (MLR). The main effect is on the responding cells, whereas when the stimulating cells are treated with Fab'‐fragments against β 2 ‐microglobulin they do not (or, at most, only slightly) change their characteristics in the MLR. Fab'‐fragments against immunoglobulin light chains do not influence the MLR significantly, whereas Fab'‐fragments against HL‐A antigens depress the response. Fab'‐fragments against β 2 ‐microglobulin do not interfere with the mitogenic stimulation of PHA, whereas Fab'‐fragments from the antiserum used in this study against HL‐A8 reduced the mitogenic effect of PHA considerably.
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Lindblom et al. (1974) studied this question.