Effects of Aralia continentalis Kitagawa on Antiplatelet and Antioxidative Activities.

독활 메탄올 추출물과 그 분획의 항혈전 및 항산화 효과를 확인하기 위하여 ADP를 이용하여 혈소판 응집억제효과를 탐색하고 DPPH 및 ABTS-radical 소거능을 측정하여 항산화능을 측정하였다. 그 결과 독활, 특히 EtOAc 분획은 ADP에 의해 유도된 혈소판응집과 트롬빈에 의한 혈소판 부착에 대하여 농도 의존적으로 저해효과를 나타내었으며, 높은 폴리페놀 함량과 가장 높은 라디칼 소거능을 통하여 독활의 항산화 효과를 확인하였다. 이상의 결과로 독활 EtOAc 분획의 높은 DPPH 및 ABTS radical 소거능이 항혈소판 효과에 영향을 미치는 것으로 사료된다. The dried roots of Aralia continentalis Kitagawa is known to have the potential for anti-inflammation and anti-rheumatic, but their effects on thrombosis are not clear. In this study, we evaluated the anti-platelet and antioxidative activities of A. continentalis Kitagawa. Methanol extract and its various fractions of A. continentalis Kitagawa inhibited ADP-induced platelet aggregation, and EtOAc fraction showed strongest inhibition in a concentration-dependent manner with a <TEX>IC₅₀</TEX> value of 217.7 <TEX>μg/ml</TEX>. Moreover, the EtOAc fraction contained 77.7% of 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 43.1% of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity at 0.1 mg/ml. In addition, the methanol and EtOAc fraction dose-dependently inhibited thrombin-stimulated platelet adhesion to collagen or fibrinogen. Collectively, these results suggest that the polyphenol-rich EtOAc fraction from A. continentalis Kitagawa can reduce platelet hyperactivation by scavenging free radicals. Thus, the EtOAc fraction of A. continentalis Kitagawa is a potential source for inhibition of platelet-dependent thrombosis.