Ultrastructure and synaptology of the nucleus ambiguus in the rat: The compact formation

The fine structure of the esophagomotor compact formation of the nucleus ambiguus was studied. Esophageal motoneurons are atypical in that they have extensive direct somato-somatic and somato-dendritic appositions without intervening glial processes. A unique feature is the presence of finger- and leaf-like somatic protrusions which partially wrap longitudinally oriented dendrites and, occasionally, small groups of dendrites and axons. The neuropil contains many longitudinally oriented, small-diameter dendrites of relatively uniform size (1.1 +/- 0.4 S.D. micrograms in diameter). Motoneuronal somatic profiles have 0-5 synapses per profile which represents a synaptic density of 10.6 synapses per soma. Axodendritic synapses measure 0.5 x 0.7 microgram in the transverse plane and are up to 3.0 micrograms long in the sagittal plane. Many axon terminals contact both a soma and dendrite in close apposition. Most axon terminals (> 90%) contain round vesicles and form asymmetric junctions with somata and dendrites. Axon terminal degeneration after electrolytic lesions and labelling after injection of wheat germ agglutinin-horseradish peroxidase in the nucleus of the tractus solitarius show that afferent connections to the compact formation form axodendritic synapses. The ultrastructure and synaptology of esophageal motoneurons is characterized by the close apposition of somata and dendrites (somatic-dendritic bundling), and the longitudinal orientation of dendrites (dendritic bundling), axons and axon terminals in the neuropil. These features may be important morphological substrates for synchronization and coordination of esophageal motoneuronal activity and esophageal peristalsis.