Quantitation by Radioimmunoassay of Thrombospondin Synthesised and Secreted by Human Endothelial Cells

Synthesis and secretion of thrombospondin by cultured human endothelial cells was quantified using a sensitive radioimmunoassay. Two endothelial lines were examined; those from umbilical veins and those from saphenous veins. The foetal line secreted approximately 17 micrograms/10(6) cells of the antigen at 24 hr, whereas the saphenous line secreted five times less in the same period. Intracellular values were similar in both lines. Thrombin and calcium ionophore A23187 were added to umbilical vein endothelial cultures. In contrast to platelets where both agents cause a rapid release reaction, no such thrombospondin release was observed in endothelial cells.