The use of the quantitative ring precipitation reaction for determining the intensity of cross reactions with special reference to comparative serology

Practical directions and examples are given for the use of quantitative ring precipitation to determine the intensity of cross reactions. The ability of this method to reproduce objectively the intensity of an antigen-antibody reacting system under various concentrations was verified. A comparison was made between Boyden’s method of "complete curves" and the quantitative ring precipitation method. The advantage of using quantitative ring precipitation is the saving of antiserum (0.05 ml. is sufficient for one determination), the smaller demand on working time and a noticeably reduced sensitivity to non-specific factors. Quantitative ring precipitation (QRP) is a suitable method for quantitative experiments in immunochemistry and for purposes of comparative serology.