Key result
CD11b-expressing aortic macrophages exhibit ~11-fold higher nanoparticle pinocytosis than non-CD11b cells.
Answers are grounded in this paper and cite their sources.
OBJECTIVE: Fluid-phase pinocytosis is a receptor-independent mechanism of endocytosis that occurs in all mammalian cells and may be a mechanism for the uptake of LDL by macrophages. As there are currently no methods for the measurement of fluid-phase pinocytosis by individual aortic cells in vivo, we sought to identify a suitable method. METHODS: ApoE-/- mice were retro-orbitally injected with AngioSPARK fluorescent nanoparticles specifically designed to not interact with cells. After 24 h, mice were sacrificed, and the aortas were isolated and then digested to analyze aortic cell uptake of AngioSPARK by flow cytometry. RESULTS: CD11b-expressing aortic macrophages from mice injected with AngioSPARK showed high levels of fluid-phase pinocytosis compared to aortic cells not expressing CD11b (4,393.7 vs. 408.3 mean fluorescence intensity [MFI], respectively). CONCLUSION: This new technique allows for the measurement of fluid-phase pinocytosis by aortic cells in vivo, making it possible to examine the cell-signaling molecules and drugs that affect this process. Published by S. Karger AG, Basel.
No takes yet. Share an insight, caveat, or question.
Anzinger et al. (2017) studied Aortic cell fluid-phase pinocytosis in ApoE-/- mice. AngioSPARK fluorescent nanoparticles in CD11b-expressing aortic macrophages vs. Aortic cells not expressing CD11b was evaluated on Fluid-phase pinocytosis measured by mean fluorescence intensity (MFI). CD11b-expressing aortic macrophages showed higher levels of fluid-phase pinocytosis of AngioSPARK nanoparticles compared to non-CD11b-expressing aortic cells (4,393.7 vs. 408.3 MFI).
Synapse has enriched 10 related papers. Consider them for comparative context: